The principle
ViennaLab Diagnostics provides the entire workflow from DNA to report generation.
Two-step PCR-based target enrichment for bacterial classification based on the 16S rRNA sequence:
- In the first PCR, regions of interest are enriched by amplification.
- The second PCR introduces specific dual-indices for subsequent sample assignment, as well as adaptors for the sequencing on Illumina platforms.
- Species-level classification of bacterial composition with ViennaLab Microbiome Analysis Webtool.