The principle

ViennaLab Diagnostics provides the entire workflow from DNA to report generation.

Two-step PCR-based target enrichment for bacterial classification based on the 16S rRNA sequence:

• In the first PCR, regions of interest are enriched by amplification.
• The second PCR introduces specific dual-indices for subsequent sample assignment, as well as adaptors for the sequencing on Illumina platforms.
• Species-level classification of bacterial composition with ViennaLab Microbiome Analysis Webtool.