Our QuickGuides facilitate the setup and the analysis of RealFast™ Assay experiments on your real-time PCR instrument.
RealFast™ Assay experiments for different applications :
- Genotyping experiments with RealFast Assays™ are designed to detect single nucleotide polymorphisms (SNPs) and enable for the distinction of the three possible genotypes (wild type/wild type, mutant/wild type and mutant/mutant) .
- Variant detection experiments detect the presence or absence of a specific variant of a gene (e.g. the HLA-B*57 variant of the HLA-B gene)
- Copy number variation (CNV) experiments measure relative quantities by comparing ∆Cq-values of the target gene and the endogenous control in relation to a calibrator. Possible applications are the detection of gene deletions or duplications.
Product notes
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Productnote 01: CVD-Marker
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Productnote 02: HLA-B27
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Productnote 03: HLA-B5701
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Productnote 04: HLA-B1502
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Productnote 05: HLA-A3101