VIENNALAB - Established innovations in diagnostics

ViennaLab Diagnostics offers a variety of easy-to-use in vitro diagnostic kits for the detection of genetic mutations, as well as genetic predispositions, pharmacogenetics and oncology.

The ViennaLab StripAssays® based on reverse hybridization carry up to 48 immobilised probes for wild-type and mutated alleles.

StripAssay® Technology Video

The principle:

1st step: Isolation of genomic DNA from bodily fluids, tissue or cells.

2nd step: Multiplex PCR amplification of specific DNA sequences and simultaneous biotin-labelling.

3rd step: Precise selective hybridization of specific sequences onto StripAssay®.

4th step: Easy and clear identification of hybridized sequences of interest only. Biotinylated sequences are detected by streptavidin-alkaline phosphatase.

5th step: Automated result interpretation possible.

The ViennaLab RealFastTM Assays are based on real-time PCR and detect single nucleotide polymorphisms (SNPs) and copy number variations (CNVs) fast and simply.

RealFastTM Technology Video

 The principle:

  • The ViennaLab RealFast™ Assays are based on real-time PCR and hydrolysis probes, also commonly called TaqMan® probes.
  • The sequence-specific oligonucleotide probes carry a fluorescent reporter dye at the 5´-end and a quencher dye at the 3´-end.
  • While the probe is intact, the quencher is close enough to the reporter to suppress the fluorescent signal of the 5´-fluorophore.
  • During the combined annealing/extension phase of PCR, the probe is cleaved by the 5´ to 3´ exonuclease activity of Taq DNA polymerase, thereby separating the fluorophore from the quencher dye.
  • This process results in detectable fluorescence, which is proportional to the amount of accumulated PCR product.